Bacterial Sex Pheromone-Induced Plasmid Transfer Minireview
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چکیده
منابع مشابه
Isolation and structure of the bacterial sex pheromone, cAD1, that induces plasmid transfer in Streptococcus faecalis.
The Streptococcus faecalis sex pheromone cAD1, which is involved in the conjugative transfer of the hemolysin plasmid pAD1, has been isolated and its structure determined. Its Mr is 818 and its amino acid sequence is H-Leu-Phe-Ser-Leu-Val-Leu-Ala-Gly-OH. A replicate of the pheromone synthesized by the liquid-phase method showed the same biological activity and chromatographic behavior as the is...
متن کاملSex-Pheromone System and Plasmid Transfer in Enterococcus feacalis
Pheromone-inducible plasmid transfer is an important mechanism for dissemination of antibiotic resistance and virulence factors in these organisms. Plasmid-free strains of Enterococcus faecalis secrete at least half a dozen peptide sex pheromone-probably many more-that induce a mating response by potential donor strains carrying members of specific families of conjugative plasmids. The response...
متن کاملTargeted disruption of the PD78 gene (traF) reduces pheromone-inducible conjugal transfer of the bacteriocin plasmid pPD1 in Enterococcus faecalis.
Bacterial sex pheromone, cPD1, induces sexual aggregation of Enterococcus faecalis harboring the bacteriocin plasmid, pPD1, and enables pPD1 to transfer at high frequency in a liquid culture. PD78 is a cPD1-inducible cell surface protein encoded by pPD1. The PD78 gene, traF, was disrupted by homologous recombination between pPD1 and an artificial vector having a deletion in the middle portion o...
متن کاملAnalysis of functional domains of the Enterococcus faecalis pheromone-induced surface protein aggregation substance.
Pheromone-inducible aggregation substance (AS) proteins of Enterococcus faecalis are essential for high-efficiency conjugation of the sex pheromone plasmids and also serve as virulence factors during host infection. A number of different functions have been attributed to AS in addition to bacterial cell aggregation, including adhesion to host cells, adhesion to fibrin, increased cell surface hy...
متن کاملControl of Enterococcus faecalis sex pheromone cAD1 elaboration: effects of culture aeration and pAD1 plasmid-encoded determinants.
Aeration of plasmid-free Enterococcus faecalis strains resulted in an 8- to 16-fold decrease in sex pheromone cAD1 activity in culture filtrates. Levels of two unrelated pheromones, cPD1 and cAM373, were unaffected by culture aeration. Aeration also resulted in a decrease in the expression of conjugative transfer functions observed in cells containing pAD1 traB mutations, verifying a link betwe...
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